Part:BBa_K1139150:Design

Prm/lac-GFP-TT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 769
  

Design Notes

No

Source

Promoter from oligo DNA, rbs from BBa_B0034, GFP from BBa_E0040, Double terminator from BBa_B0015 are combined.

References

1. Ptashne, M. (1986) A genetic switch: Gene control and phage. lambda. Other Information: From review in Mathematical Biosciences; Vol. 86, No. 2 (Oct 1987)
2. Kentaro Takei, Hitoshi Sakakibara, Mitsutaka Taniguchi and Tatsuo Sugiyama. (2001) Nitrogen-Dependent Accumulation of Cytokinins in Root and the Translocation to Leaf: Implication of Cytokinin Species that Induces Gene Expression of Maize Response Regulator. Plant Cell Physiol. 42, 85–93
3. Kenneth V. Thiman. (1937) On The Nature of Inhibitions Caused by Auxin. American Journal of Botany. Vol. 24, No. 7, 407-412
4. Basu S, Mehreja R, Thiberge S, Chen MT, Weiss R. (2003) Spatiotemporal control of gene expression with pulse-generating networks. Proc. Natl. Acad. Sci. USA, 101, 6355-6360
5. S. Mangan, S. Itzkovitz, A. Zaslaver, U. Alon. (2006) The Incoherent Feed-forward Loop Accelerates the Response-time of the gal System of Escherichia coli . J. Mol. Biol. 356, 1073-1081